Analyzing qPCR data. Using Cq values from quantstudio application. Convert to absolute quantification using standard curves
## [1] "Using Standard curve : Std30"
Largest melting temperature peak
All peaks of melting temperature
| Target_name | assay_var.label | mean_Copies.per.ul.template | Sample_category |
|---|---|---|---|
| 16s | MG1655 | 3.65e+07 | negative |
| 16s | MJD61 | 4.84e+07 | Maximal |
| 16s | MJD61 | 2.56e+07 | Repressed |
| 16s | 80a | 5.41e+07 | Maximal |
| 16s | 80a | 3.95e+07 | Repressed |
| 16s | NTC | 1.35e+04 | negative |
| 16s | AOS107 | 8.16e+06 | Maximal |
| 16s | AOS109 | 1.62e+07 | Maximal |
| U64 | MG1655 | NaN | negative |
| U64 | MJD61 | 5.30e+00 | Maximal |
| U64 | MJD61 | NaN | Repressed |
| U64 | 80a | 6.44e+01 | Maximal |
| U64 | 80a | 1.26e-02 | Repressed |
| U64 | NTC | NaN | negative |
| U64 | AOS107 | 1.23e+00 | Maximal |
| U64 | AOS109 | 8.94e-03 | Maximal |
| gfpbarcode | MG1655 | 1.18e-01 | negative |
| gfpbarcode | MJD61 | 2.45e+05 | Maximal |
| gfpbarcode | MJD61 | 2.35e+03 | Repressed |
| gfpbarcode | 80a | 2.00e+05 | Maximal |
| gfpbarcode | 80a | 1.11e+03 | Repressed |
| gfpbarcode | NTC | 3.15e+01 | negative |
| gfpbarcode | AOS107 | 6.40e+04 | Maximal |
| gfpbarcode | AOS109 | 5.06e+04 | Maximal |
for legacy use if someone prefers these. labels are not very readable
## Warning: Removed 11 rows containing missing values (`geom_point()`).
## Warning: Removed 7 rows containing non-finite values (`stat_boxplot()`).
## Removed 11 rows containing missing values (`geom_point()`).
## Warning: Removed 7 rows containing non-finite values (`stat_boxplot()`).
## Removed 11 rows containing missing values (`geom_point()`).